1. Use the search bar to navigate to other genes
The IGV search bar accepts gene names or genomic coordinates. Type a gene symbol or region into the box and press Enter, and the browser will jump directly to your region of interest.
Explore the Epitranscriptome: Healthy Mouse Cardiac Tissue
Detect, visualize, and explore m6A, inosine, and RNA expression profiles generated with the EpiPlex™ Platform from healthy mouse cardiac tissue.
Click the button below to open a pre-configured IGV session and explore epitranscriptomic patterns in mouse cardiac tissue.
EpiScout Visualization
The EpiScout report provides a comprehensive overview of epitranscriptomic modifications detected in healthy mouse cardiac tissue, including m6A and inosine modifications alongside RNA expression profiles.
Click the button below to launch the IGV genome browser with pre-configured tracks showing m6A, inosine, and RNA-seq data from male and female mouse cardiac tissue samples.
Open IGV Browser Session- Click the button above to open the IGV browser.
- Use the search bar to navigate to genes or coordinates of interest.
- Explore the m6A, inosine, and RNA-seq tracks for each sample.
2. Check the Y-axis scaling when you move to new genes
When you jump to a new region, coverage and modification tracks can span a wide dynamic range. If you don't see clear pileups or peak shapes, try right-clicking on the track to adjust the data range or enable autoscaling.
Experimental Overview
This dataset profiles epitranscriptomic signatures in healthy mouse cardiac tissue and is designed as a lightweight demo for interactive exploration.
- Sample type: Total RNA (250 ng) was extracted from healthy mouse cardiac tissue from two male and two female mice.
- Method: RNA was assayed with the EpiPlex™ Total RNA workflow to prepare m6A and inosine enriched cDNA libraries with paired RNAseq libraries. Sample libraries were then sequenced and processed with AlidaBio's EpiScout™ v1.2 pipeline for read alignment, peak-calling, and comparative analyses.
- Data tracks: The IGV session includes m6A, inosine, and RNA-seq alignment tracks for both male and female samples, along with peak calls for each sample.
Acknowledgments: Samples were prepared by the Hinson Lab at Jackson Laboratory and sent to AlidaBio for assay and analysis.
