Purchase instructions
Sequencing libraries generated with EpiPlex™ reagent kits require EpiPlex-specific Unique Dual Index (UDI) primers in the PCR step. UDI primers can be purchased during checkout or obtained independently using the oligo sequences provided on the UDI Kit product page. Access to the EpiScout™ analysis software is included with the purchase of each reagent kit. Select how you want to access the EpiScout™ software – it is available as a click-button tool on DNAnexus or as a command line tool on docker. If you chose the DNAnexus option, our support team will assist you with the DNAnexus account setup.
EpiPlex™ RNA Mod Encoding Kit
The EpiPlex™ RNA Mod Encoding Kit enables the detection and relative quantification of m6A and inosine in RNA species ≥ 100 nucleotides. The kit provides all reagents required for processing of 8 or 24 samples in a 1-day workflow. Each sample is split into an enrichment reaction and a solution control, resulting in two Illumina sequencing libraries per sample. Magnetic beads simplify solution exchanges and enable multi-channel pipetting for ease of operation and assay throughput. The included RNA modification standards maximize assay robustness, serve as in-line quality controls and enable relative quantification. EpiPlex™ Kit performance has been validated with DNAase-treated, purified RNA from a variety of sample types, including cell lines, tissue and blood. User guides for workflows using total or poly-A selected RNA as input are available for download.
EpiScout™ Analysis Software
The EpiScout™ analysis software is a companion product to EpiPlex™ kits. The end-to-end data processing platform takes FASTQ files produced by Illumina sequencing of EpiPlex libraries as an input and returns the location and abundance of m6A and inosine, along with RNA expression data. Outputs include summary tables, peak lists, gene viewer files and publication-ready plots. Using a machine learning trained peak caller and a quantification module that leverages the EpiPlex RNA modification controls, the software delivers the best results from your data and makes bioinformatics analysis accessible to all scientists. Choose between a cloud app with user-friendly GUI hosted by DNAnexus or download the docker image and run the software on your own server environment as a command line utility.
